![]() ![]() Chromosoma 100:139–146Įarnshaw WC, Bernat RL (1991) Chromosomal passengers: toward an integrated view of mitosis. Wiley-Liss, New York, pp 393–412Įarnshaw WC, Bernat RL (1991) Chromosomal passengers: toward an integrated view of mitosis. In: Hyams JS, Lloyd CW (eds) Microtubules (Modern cell biology vol 13). Genes Dev 6: 93–104Įarnshaw WC (1994) Structure and molecular biology of the kinetochore. Exp Cell Res 170:417–427ĭohrmann PR, Butler G, Tamai K, Dorland S, Greene JR Thiele DJ, Stillman DJ (1992) Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase. Proc Natl Acad Sci USA 80:2926–2930ĭavis FM, Wegner RD, Rao PN (1987) Monoclonal antibody with specificity to mitotic chromosomes of primates. J Cell Biol 122:985–992ĭavis FM, Tsao TY, Fowler SK, Rao PN (1983) Monoclonal antibodies to mitotic cells. Nature 375: 418–421Ĭoverley D, Downes S, Romanowski P Laskey RA (1993) Reversible effects of nuclear membrane permeabilization on DNA replication: evidence for a positive licensing factor. Mol Cell Biol 13: 5524–5537Ĭhong JPJ, Mahbubani HM, Khoo C-Y, Blow JJ (1995) Purification of an MCM-containing complex as a component of the DNA replication licensing system. Nature 332: 546–548īrazas RM, Stillman DJ (1993) Identification and purification of a protein that binds DNA cooperatively with the yeast SWI5 protein. J Cell Biol 122:993–1002īlow JJ, Laskey RA (1988) A role for the nuclear envelope in controlling DNA replication within the cell cycle. Biochem Biophys Res Commun 60: 1410–1417īlow JJ (1993) Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor. FEBS Lett, 297:42–45īerezney R, Coffey DS (1974) Identification of a nuclear matrix protein. J Cell Biol 108:2435–2447īelyaev ND, Budker VG, Dubrovskaya VA, Kim AA, Kiseleva EV, Sidorov VN (1992) Localization of proteins forming the outer surface of isolated metaphase chromosomes. These results suggest that LFM-1 antigens fit some of the predictions of the licensing factor model, and may have a role in cell cycle dependent events.īehrens J, Mareel MM, Van-Roy FM, Birchmeier W (1989) Dissecting tumor cell invasion epithelial acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion. They shared the sequence at the amino-terminal end but failed to show total homology with known proteins. The Mr 58000 and a minor Mr 38000 protein (which was enriched only in mitotic chromosomes of synchronized cells) were analyzed by Edman degradation. These results were independently confirmed on nuclei sorted by flow cytometry and in cell populations synchronized by release of G1-/S-phase hydroxyurea arrest. Parallel analysis of interphase nuclei revealed LFM-1 epitopes inside G1-, but excluded from G2-phase nuclei. In addition, confocal microscopy of those chromosomes revealed the LFM-1 epitopes distributed on the external surface and the axis of chromatids. Biochemical extraction procedures on isolated metaphase chromosomes from nocodazole-synchronized cells indicated that the Mr 58000 protein behaves as a chromosomal scaffold protein, that is, it remains in the pellets after high salt (2 M NaCl) or 3′–5′ diiodosalicylic acid treatments, even in DNAse pre-digested samples. Cell fractionation methods followed by immunoblotting and immunofluorescence showed that this protein is associated with the nuclear fraction. In the present work we report an Mr 58000 protein in MDCK epithelial cells, recognized by a monoclonal antibody (LFM-1) that decorates chromosomes during M-phase. Because the mechanisms that govern mitosis are a key to the understanding of cell growth, the proteins associated with chromosomes specifically during this phase have received thorough attention. ![]()
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